Composite
Part:BBa_K3832008
Designed by: Hongyu Chen Group: iGEM21_XJTU-China (2021-09-26)
Induced expression circuit of aroG (Mutant s211f)
This Part is the Induced expression circuit of CDS aroG (Mutant s211f) (BBa_K3832000).It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 76
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2469
Illegal NheI site found at 2492 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1034
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 76
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 76
- 1000COMPATIBLE WITH RFC[1000]
DETAILED INFORMATION IS SHOWN IN PAGE OF (BBa_K3832000)
[edit]
Categories
Parameters
//cds/enzyme/ligase
biology | E.coli |
kegg | EC 2.5.1.54 |
protein | aroG |