Coding

Part:BBa_K3817004:Design

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


Cdh enzyme


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2550
    Illegal PstI site found at 3103
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1642
    Illegal PstI site found at 2550
    Illegal PstI site found at 3103
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 607
    Illegal XhoI site found at 1876
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2550
    Illegal PstI site found at 3103
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2550
    Illegal PstI site found at 3103
    Illegal AgeI site found at 345
    Illegal AgeI site found at 837
    Illegal AgeI site found at 1005
    Illegal AgeI site found at 1240
    Illegal AgeI site found at 1480
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has multiple subunits, after which the sequence was subjected to directed evolution to improve its heat resistance.


Source

It comes from Pseudomonas sp. CBB1.

References