T7

Part:BBa_K3817003

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


T7 promoter-lac operator-RBS

This part is used to initiate transcription and translation, and the RBS is a highly efficient ribosome binding site extracted from gene 10 of T7. This is an essential part to ensure downstream protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None