Tag

Part:BBa_K3817002:Design

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


6His-thrombin site-SUMO tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 269
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 269
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 269
    Illegal BglII site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 269
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 269
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We need to adjust the position of this tag to ensure the best purification effect.


Source

It comes from the plasmids Pbluescript ⅡSK+.

References