Composite

Part:BBa_K3809013

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-10-19)


Modified mRFP protein under strong constitutive promoter and RBS

This sequence is the result of the assembly of two parts: BBa_K3809011 and BBa_K081005. The first one is the coding sequence of a modified mRFP1. It was modified in order to express the reporter protein alongisde other features we added to prove the correct folding of mRFP and ESR1. The sequence also includes a 6X Histidine Tag (for its purification), a linker sequence of 4 glycines, 1 serine and 1 cistein (for its immobilization) and a signal peptide sequence (NPS4). The second part is a strong constitutive promoter with an RBS with an efficiency of 0.6. We used this part for the expression of the modified mRFP protein and its subsequent purification so that we could use it to test the correct folding of the reporter, as well as to check if the immobilization in a Quartz Crystal Microbalance could be done with the characteristics we added. Overall, this sequence will aid in the project we worked on: the detection of Endocrine Disrupting Chemicals or EDCs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 112
    Illegal AgeI site found at 715
    Illegal AgeI site found at 827
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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