Translational_Unit

Part:BBa_K3803014:Design

Designed by: Shiyu Yu   Group: iGEM21_Jiangnan_China   (2021-09-02)


XDH translational unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 166
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 166
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 166
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 166
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The condon of XDH from Scheffersomyces stipitis had been optimized for better expression in S. cerevisiae.


Source

The promoter and terminator is from S. cerevisiae, and the coding gene of XDH is from Scheffersomyces stipitis.

References