CDS - Human Aryl hydrocarbon Receptor (Truncated)
This sequence encodes for the truncated version of the human Aryl hydrocarbon receptor (AhR). AhR is a transcription factor that is activated by ligands such as the 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Tryptophan metbolites such as kynurenic acid form an important class of ligands to the AhR. Upon Ligand binding, AhR translocates to the nucleus where it forms a heterodimer with the AhR Nuclear Translocator (ARNT). The heterodimer binds to the DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.
The part contains the coding sequence for a truncated version of AhR containing the the basic helix-loop-helix, PAS-A and PAS-B domains. The CDS has been codon optimized to remove any type IIS restriction enzyme sites. An N-Terminal 6x HIS tag is added to the CDS.
Sequence and Features
- 10Illegal EcoRI site found at 364
- 12Illegal EcoRI site found at 364
Illegal NheI site found at 169
- 21Illegal EcoRI site found at 364
Illegal BglII site found at 1179
- 23Illegal EcoRI site found at 364
- 25Illegal EcoRI site found at 364
- 1000COMPATIBLE WITH RFC