Coding

Part:BBa_K3793004

Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos   Group: iGEM21_Aalto-Helsinki   (2021-10-21)


CDS - Human Aryl hydrocarbon Receptor (Truncated)

This sequence encodes for the truncated version of the human Aryl hydrocarbon receptor (AhR). AhR is a transcription factor that is activated by ligands such as the 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Tryptophan metbolites such as kynurenic acid form an important class of ligands to the AhR. Upon Ligand binding, AhR translocates to the nucleus where it forms a heterodimer with the AhR Nuclear Translocator (ARNT). The heterodimer binds to the DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.

The part contains the coding sequence for a truncated version of AhR containing the the basic helix-loop-helix, PAS-A and PAS-B domains. The CDS has been codon optimized to remove any type IIS restriction enzyme sites. An N-Terminal 6x HIS tag is added to the CDS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 364
    Illegal NheI site found at 169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 364
    Illegal BglII site found at 1179
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None