- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal XhoI site found at 33
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 1
Illegal BsaI.rc site found at 36
The sequence has not been modified from the original.
Sequence was adopted from the Rong et al., 1998.
Rong M, He B, McAllister WT, Durbin RK. Promoter specificity determinants of T7 RNA polymerase. Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):515-9. doi: 10.1073/pnas.95.2.515. PMID: 9435223; PMCID: PMC18451.