RNA

Part:BBa_K3782017

Designed by: Jana Näf   Group: iGEM21_UNILausanne   (2021-10-19)


sgRNA 3

This part codes for a single-guide RNA (sgRNA) that is complemetary to the DNA sequence that needs to be cut. Cas9 will associate with the sgRNA and if the sgRNA finds its complementary sequence, Cas9 will induce a double-strand break in proximity of it, opening the DNA double helix.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None