Composite

Part:BBa_K3766161:Design

Designed by: Doriane Blaise   Group: iGEM21_Evry_Paris-Saclay   (2021-10-21)


AZT-11 expression cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2253
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2253
    Illegal AgeI site found at 299
    Illegal AgeI site found at 411
    Illegal AgeI site found at 1061
    Illegal AgeI site found at 2732
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Selection on aztreonam


Source

Directed evolution from BBa_K3766111

References