dCBD (Double Cellulose Binding Domain)
Binds strongly to microcrystalline cellulose (MCC) via non-covalent interactions. This part is an E. coli codon-optimized version of BBa_K1321340.
Usage and Biology
In our project, we included this part in the SpyCatcher002-dCBD construct for anchoring SpyTagged proteins onto an MCC matrix. Since dCBD binds to MCC, it is used to functionalize an MCC sheet with the OpdA enzyme which is part of the OpdA-SpyTag002 construct. This anchoring takes place by binding of SpyTag002 to SpyCatcher002.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 142
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC