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Part:BBa_K3760009:Design

Designed by: Ha Buer   Group: iGEM21_LZU-CHINA   (2021-10-01)


T7 promorter-sgRNA2-Synthesis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is designed for Cas13d. The T7 promoter and Direct repeat is common among different sgRNA synthesis, which means you do not need to change them while using.

Source

It does not come from genomic DNA. It is artificially synthesized.

References