Coding

Part:BBa_K3747106

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Periplasmic nitrate reductase D (napD) from Cupriavidus necator H16

This gene is part of the nap operon from C. necator H16. Nap is the first enzyme of the denitrification pathway, converting nitrate (NO3-) into nitrite (NO2-). NapD has an essential role in NapA (catalytic subunit) maturation as a dedicated chaperone protein [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] Sparacino-Watkins, C., Stolz, J. F., & Basu, P. (2014). Nitrate and periplasmic nitrate reductases. Chemical Society Reviews, 43(2), 676–706. https://doi.org/10.1039/c3cs60249d

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Categories
Parameters
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