Regulatory

Part:BBa_K374010

Designed by: Lisa Blanc Iversen, Maya Friis Kjaergaard, Annemi Jollmann, Anja Sander and Grzegorz Slodkowicz   Group: iGEM10_DTU-Denmark   (2010-10-20)

Gifsy1 anti-repressor which anti-represses the Gifsy1 repressor gogR from BBa_K374008

Gifsy1 is a temperate phage present many of the Salmonella enterica serovar Typhimurium strains. This part contains the anti-repressor from Gifsy1 called antO which inactivates the repressor from BBa_K374008 resulting in transcription from the pR[Gifsy1] promoter. AntO works by direct non-covalent binding to gogR and prevents its DNA binding activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 73
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None