Plasmid

Part:BBa_K3734023:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)


CHREBP-LUC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 895
    Illegal NotI site found at 974
    Illegal NotI site found at 2798
    Illegal NotI site found at 2975
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal BamHI site found at 2926
    Illegal XhoI site found at 2982
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1065
    Illegal NgoMIV site found at 2409
    Illegal NgoMIV site found at 2430
    Illegal NgoMIV site found at 2691
    Illegal NgoMIV site found at 3420
    Illegal NgoMIV site found at 4761
    Illegal NgoMIV site found at 5044
    Illegal AgeI site found at 2133
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 6569
    Illegal SapI.rc site found at 2315
    Illegal SapI.rc site found at 4610
    Illegal SapI.rc site found at 4820
    Illegal SapI site found at 5489


Design Notes

We need more intuitive monitoring methods.


Source

CHREBP is from human genomes. LUC is from glowworm.

References