Coding

Part:BBa_K3734009:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)


INSR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 551
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2055
    Illegal BglII site found at 8438
    Illegal BamHI site found at 2401
    Illegal XhoI site found at 809
    Illegal XhoI site found at 2566
    Illegal XhoI site found at 3579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 537
    Illegal NgoMIV site found at 2639
    Illegal AgeI site found at 7134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2267
    Illegal BsaI site found at 2577
    Illegal BsaI.rc site found at 502
    Illegal BsaI.rc site found at 744
    Illegal BsaI.rc site found at 1319
    Illegal BsaI.rc site found at 1775
    Illegal BsaI.rc site found at 2097
    Illegal BsaI.rc site found at 3674
    Illegal BsaI.rc site found at 3849
    Illegal SapI.rc site found at 3856
    Illegal SapI.rc site found at 9122
    Illegal SapI site found at 790
    Illegal SapI site found at 8692


Design Notes

We need to choose correct sequnce and ensure it can transport and fix in the membrane.


Source

INSR come from human genome.

References