RNA

Part:BBa_K3658034

Designed by: Yujeong Oh   Group: iGEM20_NYU_Abu_Dhabi   (2020-10-27)


Bsal guide RNA for CRISPR Cas12a

Batrachochytrium salamandrivorans (Bsal) single guide RNA (sgRNA) used for CRISPR Cas12a. The guide RNA is a complementary sequence to the target DNA. The guide RNA helps the Cas enzyme to recognize the target sequence and activates the Cas enzyme. We used DETECTR (DNA Endonuclease-Targeted CRISPR Trans Reporter), which is a novel technique that combines isothermal amplification by RPA and detection of target DNA sequence with CRISPR-Cas12a systems. Guide was attached to a LbCas12a specific scaffold.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None