Primer

Part:BBa_K3658031

Designed by: Yujeong Oh   Group: iGEM20_NYU_Abu_Dhabi   (2020-10-26)


Batrachochytrium dendrobatidis (Bd) ITS region qPCR reverse primer

Quantitative polymerase chain reaction (qPCR) used as the golden standard to compare to our results with RPA and LAMP. The ITS (internal transcribed spacer) region is highly conserved and widely used as the DNA barcode region in fungi. qPCR allows the quantification and detection of the specific DNA sequence in real time. It measures the concentration of probes or fluorescent dyes while the synthesis process is taking place.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/part
Parameters
None