Primer

Part:BBa_K3658021

Designed by: Michelle Hughes   Group: iGEM20_NYU_Abu_Dhabi   (2020-10-26)


LAMP F3 for Batrachochytrium dendrobatidis (Bd) ITS region

LAMP forward primer used to allow for amplification of Bd ITS region by the stand displacing DNA polymerase. LAMP stands for Loop-mediated AMPlification and it is a technique that amplifies DNA at a constant temperature lower than for PCR. The ITS (internal transcribed spacer) region is highly conserved and widely used as the DNA barcode region in fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None