Plasmid_Backbone
pET9d-SYB6

Part:BBa_K3653014

Designed by: Shanny Ackerman, Ella Samuel   Group: iGEM20_Technion-Israel   (2020-10-12)


pET9D with Sybody 68

pET9D is bacterial expression vector with KAN antibiotic resistance and T7 promoter. Sybodies are small-sized synthetic nanobodies (camelid single-domain antibodies) with a specific and high affinity to only one cognate target. Normally they are easy to produce, stable, soluble in aqueous solutions and can refold without additional improvements. These specific Sybodies target the receptor-binding domain (RBD) of the SARS-CoV-2 spike proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal XbaI site found at 951
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal NheI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal BglII site found at 1009
    Illegal BamHI site found at 510
    Illegal XhoI site found at 3915
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal XbaI site found at 951
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal XbaI site found at 951
    Illegal NgoMIV site found at 1041
    Illegal NgoMIV site found at 1409
    Illegal NgoMIV site found at 1569
    Illegal NgoMIV site found at 1923
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None