Plasmid_Backbone
pBSC1-Lacz

Part:BBa_K3653008

Designed by: Shay Bamany, Shany Greenstein, Dor Josef, Amir Betker   Group: iGEM20_Technion-Israel   (2020-10-11)


pBSC1_Lacz

pBSC1_Lacz can be linearized and integrated into α‐amylase (amyE) locus in B. subtilis. The plasmid contains LacUV5 promoter for membrane protein overexpression in E.coli, and a red fluorescent protein (mRFP) that serves as a reporter protein. This plasmid also contains resistance to ampicillin, carbenicillin, and. Repressor of primer (ROP) gene which encodes ROP protein, was recruited for maintaining the plasmids at low copy number.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Illegal suffix found at 1092
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1093
    Illegal PstI site found at 1107
    Illegal NotI site found at 7
    Illegal NotI site found at 1100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal BglII site found at 9514
    Illegal BamHI site found at 1113
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Illegal suffix found at 1093
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1093
    Illegal PstI site found at 1107
    Illegal NgoMIV site found at 5653
    Illegal NgoMIV site found at 7979
    Illegal NgoMIV site found at 9889
    Illegal AgeI site found at 803
    Illegal AgeI site found at 915
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 5829
    Illegal SapI.rc site found at 6911


[edit]
Categories
Parameters
None