Coding

Part:BBa_K3651004

Designed by: Zoe Hsieh, Maria Lyons, Josh Dayie, Max Swann   Group: iGEM20_Baltimore_BioCrew   (2020-09-26)


Codon optimized feoB gene for Synechococcus sp. CB0101 with restriction uptake sites removed

FeoB is found in many bacteria species, and the gene is best-documented as it occurs in E. coli. However, it is also (likely) a critical iron uptake gene for Synechocystis sp. PCC6803. It is one of three parts of the Ferrous Iron Transport system--FeoB is a large membrane protein that is capable of hydrolysing GTP. It is useful to bacteria living in anaerobic or low pH conditions where the Fe2+ form of iron is dominant. It should be noted that FeoB is part of a system, and its efficacy may depend on the presence of FeoA and FeoC.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 202
    Illegal PstI site found at 349
    Illegal PstI site found at 442
    Illegal PstI site found at 1181
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 202
    Illegal PstI site found at 349
    Illegal PstI site found at 442
    Illegal PstI site found at 1181
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 202
    Illegal BamHI site found at 1456
    Illegal XhoI site found at 949
    Illegal XhoI site found at 1082
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 202
    Illegal PstI site found at 349
    Illegal PstI site found at 442
    Illegal PstI site found at 1181
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 202
    Illegal PstI site found at 349
    Illegal PstI site found at 442
    Illegal PstI site found at 1181
    Illegal NgoMIV site found at 1065
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1012
    Illegal BsaI.rc site found at 613


[edit]
Categories
Parameters
None