Yarrowia lipolytica LEU4 gene
2-isopropylmalate (LEU4) coding sequence from Yarrowia lipolytica .
Usage and Biology
2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway (1). The protein has a leucine binding domain which allows for downregulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate leucine synthesis and produce a leucine overproducing strain.
Below is the codon-optimized sequence of Yarrowia lipolytica LEU4 for use in any expression construct. However, we have also made our own expression construct BBa_K3629023, designed to allow for optimal expression of LEU4 in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 728
Illegal BamHI site found at 293
Illegal XhoI site found at 428
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 799
- 1000Illegal BsaI.rc site found at 544
1. KEGG PATHWAY: Valine, leucine and isoleucine biosynthesis. (n.d.). Retrieved October 26, 2020, from https://www.genome.jp/kegg-bin/show_pathway?yli00290