Coding

Part:BBa_K3590032:Design

Designed by: Barbora Hrnčířová   Group: iGEM20_GenerationMendelBrno   (2020-10-18)


Linker 2 version 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized for expression in Bacillus subtilis and a few codons have been changed in version 2 (BBa_K3590033) to avoid homologous recombination between the linkers in one construct.


Source

Source: The original sequence of L2 was taken from the Scaf19L sequence (where it is called Linker 3) published in the article by Vazana and colleagues (2013). The sequence was codon-optimized for expression in Bacillus subtilis and a few codons have been changed in version 2 to avoid homologous recombination between the linkers in one construct.

Vazana Y., Barak Y., Unger T., Peleg Y., Shamshoum M., Ben-Yehezkel T., Mozor Y., Shapiro E., Lamed R. and Bayer E. A. 2013. A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates. Biotechnol. Biofuels 6: 182. DOI: 10.1186/1754-6834-6-183

References