Coding

Part:BBa_K3590030:Design

Designed by: Barbora Hrnčířová   Group: iGEM20_GenerationMendelBrno   (2020-10-18)


Linker 1 version 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized for expression in Bacillus subtilis and a few codons have been changed in versions 2, 3 and 4 to avoid homologous recombination between the linkers in one construct. Further versions: BBa_K3590028, BBa_K3590029, BBa_K3590031


Source

The original sequence of L1 was taken from the Scaf19L sequence (where it is called Linker 2) published in the article by Vazana and colleagues (2013). The sequence was codon-optimized for expression in Bacillus subtilis and a few codons have been changed in versions 2, 3 and 4 to avoid homologous recombination between the linkers in one construct.

Vazana Y., Barak Y., Unger T., Peleg Y., Shamshoum M., Ben-Yehezkel T., Mozor Y., Shapiro E., Lamed R. and Bayer E. A. 2013. A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates. Biotechnol. Biofuels 6: 182. DOI: 10.1186/1754-6834-6-183

References