Coding

Part:BBa_K3590020:Design

Designed by: Barbora Hrnčířová   Group: iGEM20_GenerationMendelBrno   (2020-10-18)


3x LysM domain derived from Bacillus subtilis LytE version 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 325
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 325
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 387


Design Notes

The sequence was codon-optimized for easier gene synthesis.


Source

Accession: U38819, nucleotides 515 to 1132. The sequence was codon-optimized for easier gene synthesis and a few codons have been changed in version 2 to avoid homologous recombination between the linkers in one construct.

References