Coding

Part:BBa_K3590016:Design

Designed by: Barbora Hrnčířová   Group: iGEM20_GenerationMendelBrno   (2020-10-18)


Cohesin derived from Bacteroides cellulosolvens


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 323
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 323
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized for expression in Bacillus subtilis.


Source

The original sequence of CohBc was taken from the Scaf19L sequence published in the article by Vazana and colleagues (2013). The sequence was codon-optimized for expression in Bacillus subtilis.

Vazana Y., Barak Y., Unger T., Peleg Y., Shamshoum M., Ben-Yehezkel T., Mozor Y., Shapiro E., Lamed R. and Bayer E. A. 2013. A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates. Biotechnol. Biofuels 6: 182. DOI: 10.1186/1754-6834-6-185

References