Coding

Part:BBa_K3590013:Design

Designed by: Barbora Hrnčřov   Group: iGEM20_GenerationMendelBrno   (2020-09-11)


Cellulose binding module


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized for expression in Bacillus subtilis.


Source

The original sequence of CBM was taken from the Scaf19L sequence published in the article by Vazana and colleagues (2013). Vazana Y., Barak Y., Unger T., Peleg Y., Shamshoum M., Ben-Yehezkel T., Mozor Y., Shapiro E., Lamed R. and Bayer E. A. 2013. A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates. Biotechnol. Biofuels 6: 182. DOI: 10.1186/1754-6834-6-182

References