Other
I2-10

Part:BBa_K3583001

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


I2-10 Promoter for Physcomitrella patens

This part is a highly efficient promoter for the moss Physcomitrella patens. We found the promoter in a paper and asked the author for the sequence.

Source

Peramuna A, Bae H, Rasmussen EK, Dueholm B, Waibel T, Critchley JH, Brzezek K, Roberts M, Simonsen HT. Evaluation of synthetic promoters in Physcomitrella patens. Biochem Biophys Res Commun. 2018 Jun 2;500(2):418-422. doi: 10.1016/j.bbrc.2018.04.092. Epub 2018 Apr 19. PMID: 29660341.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 121
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 353
    Illegal XhoI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None