Composite

Part:BBa_K358021:Design

Designed by: Ken Kajita   Group: iGEM10_Kyoto   (2010-10-16)

Lysisbox -type2-


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


Design Notes

Lactose promoter must be repressed, even though the anti-killergene is expressed constitutively. So, we used low copy plasmid, pSB4K5, and transformed it to KRX, not Top 10. KRX is able to repress the lactose promoter. See KRX genotype[1].

Source

Used BBa_K358018 and BBa_K358006.

References