- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 1005
Lactose promoter must be repressed, even though the anti-killergene is expressed constitutively. So, we used low copy plasmid, pSB4K5, and transformed it to KRX, not Top 10. KRX is able to repress the lactose promoter. See KRX genotype.
Used BBa_K358018 and BBa_K358006.