Part:BBa_K3579016
EpiFlex_pPBEF_bb0
This plasmid is the backbone of the level 0 plasmid of the EpiFlex MoClo toolkit for S. epidermidis.
It is a E. coli -> S. epidermidis shuttle vectors. This means that they contain origins of replication for both E. coli and S. epidermidis. It has a E. coli Ampicillin resistance gene.
The level 0 plasmid aims to contain an individual part of TU. The insertion of the part in this backbone is done by golden gate assembly : This backbone has level 0 fusion sites and BbsI restriction sites allowing the part insertion. A GFP dropout cassette is present in between the the pairs of level 0 fusion sites and BbsI restriction sites allowing to verify the insertion of the part. The removal of the GFP cassette is a visual indicator of successful transformants, as the lack of a green suggests successful interruption of the cassette.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 1634
Illegal SpeI site found at 643
Illegal SpeI site found at 2442 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NheI site found at 6323
Illegal SpeI site found at 643
Illegal SpeI site found at 2442 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BamHI site found at 628
Illegal XhoI site found at 621 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 1634
Illegal SpeI site found at 643
Illegal SpeI site found at 2442 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 1634
Illegal SpeI site found at 643
Illegal SpeI site found at 2442
Illegal NgoMIV site found at 6471
Illegal AgeI site found at 980
Illegal AgeI site found at 5940 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 3785
| chassis | S. epidermidis |