RBS

Part:BBa_K3463003

Designed by: Sara Salhi   Group: iGEM20_Grenoble_Alpes   (2020-10-22)


RBS eGFP and RhlR (RhlR construction)

The design of this sequence was based on the Sang Woo Seo et al. 2012 article to modulate the expression of the following genes. This is a high RBS for the eGFP gene (BBa_K1094400) with a translation score of 228725.51, and a high RBS for the RhlR gene (BBa_C0171) with a translation score of 5242756.15. This RBS will be used coupled to eGFP reporter gene as a control for the RhlR construction BBa_K3463007 and coupled to the RhlR gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None