Plasmid_Backbone

Part:BBa_K3463000

Designed by: Iryna Moskalevska   Group: iGEM20_Grenoble_Alpes   (2020-10-22)


pUCBB-eGFP

This plasmid is a vector backbone for bacterial transformations. It is a vector for high copy bacterial expression. It contains a selection gene that encodes for the AmpR ampicillin resistance gene. It contains an eGFP (Enhanced Green fluorescent protein) (BBa_K1094400) insert under the control of a constitutive lac promoter. It contains as well a His tag at the C terminal of the protein.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2116
    Illegal XbaI site found at 2134
    Illegal SpeI site found at 3152
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2116
    Illegal SpeI site found at 3152
    Illegal NotI site found at 3069
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2116
    Illegal BglII site found at 2272
    Illegal XhoI site found at 3077
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2116
    Illegal XbaI site found at 2134
    Illegal SpeI site found at 3152
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2116
    Illegal XbaI site found at 2134
    Illegal SpeI site found at 3152
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1155


[edit]
Categories
Parameters
None