Coding

Part:BBa_K3317069

Designed by: Jorge Luis Hernandez Ayala   Group: iGEM19_UANL   (2019-10-20)


ccaSm3*

Miniaturized version of the fusion protein of light responsive domains of CcaS from Synechocystis sp. For the use as a green light activated sensor in E. coli. Miniaturized sensor kinase miniCcaS#3, with its first 15 codons optimized. Castillo-Hair, S., Baerman, E., Fujita, M., Igoshin, O., & Tabor, J. (2019). Optogenetic control of Bacillus subtilis gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1400
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None