Composite

Part:BBa_K3317059

Designed by: Jorge Luis Hernandez Ayala   Group: iGEM19_UANL   (2019-10-16)


NucE lysis construction

Optimized RBS + CDS. The RBS was designed specifically for NucE overexpression in E.coli K12, allowing extracellular secretion of the S. marcescens extracellular nuclease, inducing lysis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 237
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 237
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 241
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 237
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 237
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
//biosafety/kill_switch
//cds/lysis
//cds/membrane/lysis
Parameters
None