Generator

Part:BBa_K3317032

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-18)


Converts vanillic acidcis to muconic acid within BMCs

operon corresponding to the production of Vanillate O-demethylase (BBa_K3317001),Protocatechuate decarboxylase (BBa_K3317002) and Catechol 1,2-dioxygenase (BBa_K3317003) carrying a signal peptide to be introduced into bacterial microcompatments (BMC) under the vanillinic acid sensitive pVanCC promoter (BBa_K3317006)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4220
    Illegal BamHI site found at 4
    Illegal BamHI site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1974
    Illegal NgoMIV site found at 2046
    Illegal NgoMIV site found at 3101
    Illegal NgoMIV site found at 3882
    Illegal AgeI site found at 134
    Illegal AgeI site found at 460
    Illegal AgeI site found at 1746
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4104
    Illegal SapI.rc site found at 1124
    Illegal SapI.rc site found at 2658


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Categories
Parameters
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