Generator

Part:BBa_K3317024

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-15)


Vanillate O-demethylase (LigM gene) with the pduP sp Protein Generator

produces Vanillate O-demethylase BBa_K3317001 under a constitutive promoter BBa_J23100 cloned into pSB1C3 taking advantage of the downstream terminator of the MSC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 437
    Illegal NgoMIV site found at 1218
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1440


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Categories
Parameters
None