Coding

Part:BBa_K3317003

Designed by: Erick Hernandez, Abigail Torres and Shakti Delgadillo   Group: iGEM19_UANL   (2019-10-15)


Catechol 1,2-dioxygenase (CatA) from Pseudomonas putida

Catechol 1,2-dioxygenase (CatA) converted catechol into cis,cis-muconic acid. The enzyme was modified with a signal peptide at its N-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et. al 2017) This is a dimeric enzyme


Wild type enzyme information: E. C. 1.13.11.1

Km 0.00185

Kcat 19.39725


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
    Illegal NgoMIV site found at 355
    Illegal AgeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 967


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Categories
Parameters
None