Coding

Part:BBa_K3292000

Designed by: Abril Diosdado hernndez   Group: iGEM19_Tec-Monterrey   (2019-10-16)


Sialidase from M. viridifaciens.

This part comes from Micromonospora viridifaciens and was optimized for its expression in E. coli. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 626
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1891
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 551
    Illegal BsaI.rc site found at 415
    Illegal BsaI.rc site found at 1552


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Categories
Parameters
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