Generator

Part:BBa_K325909:ArabinosetoLight

Designed by: Theo Sanderson and Will Handley   Group: iGEM10_Cambridge   (2010-10-23)
Input: L-Arabinose
Output: Light

pBad/araC
I0500
PoPS to Light
Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        H-NS mutants        Add Data       


Description
This page describes the light output of BBa K325909 as a fucntion of Arabinose concentration in the media. The characterisation has been made in different chassis. TOP 10 cells as well as 2 mutants in which expression of H-NS was repressed.


Data

Figure 1 - Light output of BBa_K325909 as a function of Arabinose concentration in the media. The values correspond to the peak intensity within 5 hours of adding Arabinose to the media. Data points and error bars correspond to the mean and the standard deviation of 3 time repeats.
Figure 2 - Evolution of luminescence with time at different Arabinose concentrations for BBa_K325909. Measurements were taken every 30 minutes. Data points and error bars correspond to the mean and standard deviation of 3 time repeats.
Data Notes Date Uploaded
Media:BBa_K325909ArabinosetoLight.xls Raw data from experiment 21/10/2010


Measured by the Cambridge iGEM team 2010

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen), BW25113 DELTA H-NS::kan and H-NS mutant JM 230 H-NS -205::tn10.
Plasmids: Device has been shown to work on pSB1C3


References
[1]: J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.

[2]: E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176. [3]: E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.