Composite

Part:BBa_K3258026

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


MDV + Ampicillin with 2 Stop Codons

Ampicillin resistance gene with 2 stop codons, as shown in Part:BBa_K3258021, with MDV regions added upstream and downstream (Part:BBa_K3258015 and Part:BBa_K3258016), designed to be used with the DiCE evolution system. The MDV regions allow the RNA of the Ampicillin to be replicated by Qbeta, which should theoretically introduce mutations into the RNA sequence for Ampicillin and under selective pressure, eventually re-evolve working Ampicillin resistance via the RNA. A construct for testing the feasibility of the DiCE System with Qbeta in vivo.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
    Illegal NotI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal BglII site found at 1102
    Illegal XhoI site found at 168
    Illegal XhoI site found at 1096
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1105
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1105
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None