RNA

Part:BBa_K3258022

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-20)


Scr Scramble Non-targeting gRNA Negative Control

Scr (Scramble RNA) is a negative control guide RNA for testing CRISPR Cas9 construct systems. It contains a nonsense targeting sequence to generate nonsense guide RNAs in opposition to correctly targeting guide RNA controls. Was used as a negative control for RFP knockdown experiments with CRISPR Cas9 and AntiCRISPR proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 226
    Illegal BsaI site found at 404


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Categories
Parameters
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