Coding

Part:BBa_K3258007

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


M13 pIII neg

Dominant negative mutant of phage gene III that inhibits viral production when produced. Can be implemented as part of a negative selection schema.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 754
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None