Regulatory

Part:BBa_K3221201

Designed by: Yaxin Luo   Group: iGEM19_SCU-China   (2019-10-04)

the MET3 promoter(pMET3) from Saccharomyces cerevisiae

MET3 promoter(pMET3) is a tight promoter which comes from Saccharomyces cerevisiae BY4742. The pMET3 regulates MET3 gene expression, which is a vital enzyme in the methionine biosynthetic pathway in Saccharomyces cerevisiae. This promoter can be regulated by methionine. The high concentration of methionine can inhibit the promoter’ s expression, and it can turn on when the methionine in the medium at a low concentration. Because of this characteristic, we used it in our delay expression system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 399
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Categories
Parameters
None