Intermediate

Part:BBa_K3221101

Designed by: lingfeng mao   Group: iGEM19_SCU-China   (2019-09-30)


GAL1-T7 +GAL1-T7 + cns2 +CYC1

This part functions as expressing gene cns2 which is regulated by GAL1 and T7 promoter. Also it has redundant GAL1 and T7 promoter reserved for further construction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 624
    Illegal XbaI site found at 2169
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 624
    Illegal NheI site found at 545
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 624
    Illegal BamHI site found at 609
    Illegal XhoI site found at 2163
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 624
    Illegal XbaI site found at 2169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 624
    Illegal XbaI site found at 2169
    Illegal AgeI site found at 70
    Illegal AgeI site found at 699
    Illegal AgeI site found at 2232
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None