Composite

Part:BBa_K3221007

Designed by: Qihan Jin   Group: iGEM19_SCU-China   (2019-10-03)


pGAL1 - CNS3 - tCYC1

We constructed this part on YES2 plasmid to express CNS3 protein in the yeast BY4742.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3169
    Illegal SpeI site found at 1830
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1858
    Illegal SpeI site found at 1830
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2302
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3169
    Illegal SpeI site found at 1830
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3169
    Illegal SpeI site found at 1830
    Illegal AgeI site found at 150
    Illegal AgeI site found at 3232
  • 1000
    COMPATIBLE WITH RFC[1000]


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