Regulatory

Part:BBa_K3221004

Designed by: Jia Yan   Group: iGEM19_SCU-China   (2019-10-01)


TPS1 is a weak low-glucose-inducible promoter, riginally found in saccharomyces cerevisiae.

TPS1 is a weak promoter, which showes no different responce on sucrose. The constitutive expression of certain proteins and metabolic pathways can be detrimental to cell growth due to product toxicity, so you can use a weak promoter to reduce the production of proteins to reach a balance of cell's growth and the yield of interest proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 599
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 599
    Illegal NotI site found at 605
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 599
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 599
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 599
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None