Composite
Part:BBa_K3206006
Designed by: Matthew Rogan Group: iGEM19_Newcastle (2019-10-15)
Constituitively expressed ChaC gene
This composite part consists of a BBa_J23100 promoter which allows constitutive expression of the gene ChaC. ChaC encodes a glutathione-specific gamma-glutamylcyclotransferase, which catalyses the cleavage of glutathione into its main component parts (5-oxo-L-proline and cysteine-glycine dipeptide). This part can be used for glutathione degradation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 196
Illegal AgeI site found at 263 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/prokaryote/ecoli
transcriptional
transcriptional
biology | Escherichia coli K12 |
protein | ChaC |