Generator
S + P25 +

Part:BBa_K320014

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-24)

S + P25 + P28 + Ori + Tet

Constitutive expression of P25 and P28 (driven by BBa_K320002), replication origin for Asaia and E. coli and a resistance to Tetracycline .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
    Illegal NheI site found at 827
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2699
    Illegal AgeI site found at 2859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1537


[edit]
Categories
Parameters
None