Regulatory

Part:BBa_K3196036

Designed by: Xiunan Huo   Group: iGEM19_HUST-China   (2019-10-18)


AOX1

Characterization

It is a strong promoter induced by methanol and is often used to produce recombinant protein in Pichia pastoris expression system.

Usage and Biology

AOX1 gene is the main source of alcohol oxidase (Aox). AOX1 promoter is strictly induced by methanol and inhibited in the presence of glucose, glycerol, fructose and other carbon sources.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None